The development of subhas createddiffraction limit fluorescence microscopy
to visualize blood flow in the vasculature of zebrafish embryos , without using exogenous labels(passive) has been createdMicroscopy A phase variance optical coherence microscope ( pvOCM
Extra scatteringcan causeout - of phase scattering which will eliminate the diffraction
acoustic waves ... necessaryto causediffraction of light traveling within the waveguide
the limits(passive) set bythe limits
in a resolution of ~20 nm laterallyresultingin a resolution of ~20 nm laterally
the production of two diffracted cones of light at two slightly different angles , which however , have a common region of overlapcausesthe production of two diffracted cones of light at two slightly different angles , which however , have a common region of overlap
a rectangular shape modulation of the wave - front phasecreatea rectangular shape modulation of the wave - front phase
the shortest achievable distance to approximately half the wavelength of light ( > 200 nmsettingthe shortest achievable distance to approximately half the wavelength of light ( > 200 nm
the lateral resolution of optical images(passive) is set bythe lateral resolution of optical images
conventional light microscopes from resolving things smaller than half the size of the wavelength of the light , which for visible light is about 300 nanometerspreventsconventional light microscopes from resolving things smaller than half the size of the wavelength of the light , which for visible light is about 300 nanometers
to overestimations of bacterial size54 , 55leadto overestimations of bacterial size54 , 55