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using Primer Express software version 3.0 ( Applied Biosystems , Foster City , CA(passive) were designedPrimers for qRT - PCR
using Primer Express Software Version 3.0 ( Applied Biosystems , USA(passive) were designedPrimers for qRT - PCR
on the basis of the sequences derived from the deep sequencing analysis(passive) were designedPrimers used in PCR
using Primer Express software ( Applied Biosystems(passive) were designedThe primers for PCR
using the Primer Express software ( ABI(passive) were designedPrimers for qRT - PCR
using Primer Expression software ( PE - Applied Biosystems(passive) were designedThe primers for PCR
using the Primer - BLAST software developed at NCBI(passive) were designedPrimers for PCR
using Primer(passive) were designedPrimers for RT - PCR
using the PRIMER3 software ( Applied Biosystem , Massachusetts , USA(passive) were designedPrimers for the qRT - PCR
Beacon Designer 7 software ( Premier Biosoft International(passive) were designed byPrimers for qRT - PCR
where possible to overlap the site of the oligo used on the array and qRT - PCR carried out on cDNA made from RNA from the same tissue samples as used for the array experiments(passive) were designedPrimers for qRT - PCR
using Primer3 software(passive) were designedPrimers for QRT - PCR
based on the franking regions of the remnant sequences of mutY detected in the Rma genome and their corresponding sequences in Vok ( Table 2(passive) were designedPrimers for PCR of mutY
for these and quantitative RT - PCR(passive) were designedPrimers for RT - PCR
using Primer3 software(passive) were designedPrimers for PCR
using PerlPrimer software(passive) were designedPrimers for qRT - PCR
to amplify exons(passive) were designedPrimers for RT - PCR
using Primer3(passive) were designedPrimers for qRT - PCR
with PrimerBLAST software ( NCBI(passive) were designedPrimers for RT - PCR
were usedto designprimers for RT - PCR
with Primer3 software [ 30(passive) were designedPrimers for qRT - PCR
to flank each restriction site and generate PCR amplicons of 800 bp(passive) were designedPrimers for PCR
The conserved regions of plant TLPs and the N - terminal sequence were usedto designprimers for PCR
from the sequence of the putative CP1(passive) were designedPrimers for RT - PCR
using j5 online software(passive) were designedPrimers for PCR
using the Primer3 software program ( 45(passive) were designedPrimers for PCR
using Primer3 server ( ver . 4.0.0(passive) were designedPrimers for qRT - PCR
to span introns(passive) were designedPrimers for RT - PCR
for subsequent sequencing and analysis of cytosines within the amplicon(passive) are designedPrimers for Bisulfite PCR
as follows(passive) were designedPrimers for RT - PCR
using DNAstar 4.02 software(passive) were designedPrimers for the PCR
computer analysis(passive) were designed byPrimers for RT - PCR
for the coding region of the UCP1 gene(passive) were designedPrimers for PCR
from the sequence of the genomic clones(passive) were designedPrimers for PCR
to amplify the consensus sequence for Bcl-2 ( F(passive) were designedPrimers for PCR
The Primer Designer helpsdesignprimers for PCR
from the ONNV structural E1 gene ( E1 ) and non - structural protein 1 gene ( nsP1 ) ( Table 1(passive) were designedPrimers for RT - PCR
according to the chosen DNA assembly method(passive) were designedPrimers for PCR
based on the respective published DNA sequences(passive) were designedPrimers for PCR
from the nucleotide sequences available in the art(passive) can be designedPrimers for PCR
according to the expected insertion allele of the AtYFGwill be designedaccording to the expected insertion allele of the AtYFG
for each speciesdesignedfor each species
to detect these junctionsdesignedto detect these junctions
to spandesignedto span
specifically for bacterial plasmid sequencesdesignedspecifically for bacterial plasmid sequences
to detect and measure RNAis designedto detect and measure RNA
in a short PCR product of 900 bp , which is too short to be used in Tyramide - FISH experiments on Rosaresultedin a short PCR product of 900 bp , which is too short to be used in Tyramide - FISH experiments on Rosa
in our laboratorydesignedin our laboratory
with 5 degree cooling increments every 30 secondssetwith 5 degree cooling increments every 30 seconds
in the conserved kinase domain of SERK geneswere designedin the conserved kinase domain of SERK genes
Two oligonucleotides(passive) were designedTwo oligonucleotides
with the programhad been designedwith the program
for the assemblywere designedfor the assembly
for the assemblywere designedfor the assembly
to 356 bpleadsto 356 bp
in artefactsresultingin artefacts
IIIsetIII
ACCDF and ACCDRresultedACCDF and ACCDR
3set3
3 sets of primerto design3 sets of primer
Primers(passive) were designedPrimers
mutations(passive) caused bymutations
alsocontributesalso
for genotypingdesignedfor genotyping
using primers FWD_Tn5_gt , REV_Tn5_gt , FWD_Tn5/7_gt , and REV_Tn5/7_gtresultusing primers FWD_Tn5_gt , REV_Tn5_gt , FWD_Tn5/7_gt , and REV_Tn5/7_gt
to amplify three different C. pseudotuberculosis genomic regions , including a fraction of the gene responsible for phospholipase D exotoxin expressionwas designedto amplify three different C. pseudotuberculosis genomic regions , including a fraction of the gene responsible for phospholipase D exotoxin expression
The PX1 PCR plate sealer and heat seals from Bio - Rad Laboratories(passive) are designedThe PX1 PCR plate sealer and heat seals from Bio - Rad Laboratories
to detect DPYD IVS14 + 1Gdesignedto detect DPYD IVS14 + 1G
in 1983inventedin 1983