Loading ...
overabundance of template(passive) caused bysmears after PCR
to amplify 300- to 400-bp fragments that included the predicted CDREs shown in supplemental Table S2(passive) were designedOligonucleotides for PCR
Excess enzyme in your PCRcan leadto smearing of PCR products
to amplify 50to 100-bp fragments ( Table S1(passive) were designedOligonucleotides for PCR
using Primer3 ( http://frodo.wi.mit.edu /(passive) were designedOligonucleotides for PCR
either directly using the Primer3 software [ 52 ] or via the program CODDLE(passive) were designedOligonucleotides for PCR
to incorporate restriction enzyme recognition sites at their respective 5′ ends(passive) were designedOligonucleotides for PCR
either directly using the Primer3 software or via the program CODDLE(passive) were designedOligonucleotides for PCR
the rd29Adesignedprimers in PCR
to unique , gene - specific regions for each of the three genes(passive) were designedOligonucleotides for PCR
any mutations(passive) caused byany mutations
from too much template or too much polymeraseoften resultsfrom too much template or too much polymerase
to amplify regions specifically associated with the rearrangementsdesignedto amplify regions specifically associated with the rearrangements
to anneal to opposite strands of template DNAare designedto anneal to opposite strands of template DNA
up and amplification in one roomsetup and amplification in one room
of installment payments on your 5 microliter of DNA templatecomposedof installment payments on your 5 microliter of DNA template
on the basis of amanitins ( n=13 ) and phallotoxins ( n=5 ) genes in 18 species of poisonous mushrooms deposited to Genbank of the National Center for Biotechnology Informationwere designedon the basis of amanitins ( n=13 ) and phallotoxins ( n=5 ) genes in 18 species of poisonous mushrooms deposited to Genbank of the National Center for Biotechnology Information
DNA copiesto createDNA copies
parasitic disease cases(passive) caused byparasitic disease cases