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The nucleotide sequences of the cofactor CF7 and CF8 or their complements can be usedto designprimers for a polymerase chain reaction
using Primer3 Software13 ( Table 1(passive) were designedPrimer sets for polymerase chain reaction
to discriminate between mutant and wild - type alleles of glucocerebrosidase and to allow separation from products of the related pseudogene(passive) were designedPrimers for the polymerase chain reaction
The sequences of the highly conserved S4 regions of voltage - sensitive ion channels were usedto designoligonucleotide primers for the polymerase chain reaction
a blocking moietypreventspolymerase mediated chain extension on the primer template
using the design tool Oligo 6.0(passive) were designedPrimer sets for polymerase chain reaction ( PCR
the barley sequences were usedto designoligonucleotide primers for the polymerase chain reaction
In the scenario depicted in the upper panel , the perfectly complementary PNA will preferentially bind to the target wild - type genomic DNApreventingthe polymerase chain reaction primer from annealing
Sci . , 86 : 5743 ( 1989 ) ) was usedto createoligonucleotide primers for polymerase chain reaction
orderto designprimer sets for polymerase chain reaction
allele dropout and other genotyping failuresmay causeallele dropout and other genotyping failures
in antibody frequencies with only 42 to 62 % accuracyresultedin antibody frequencies with only 42 to 62 % accuracy
The PCR amplifications were carried out in a 50 μl reaction mixture containing 1X buffer , 200 μM dNTP , 0.2 μM of each primer , 2 μl DMSO , 200 ng genomic DNA templates , and 2 units of thermostable polymerase ( proTagsetsThe PCR amplifications were carried out in a 50 μl reaction mixture containing 1X buffer , 200 μM dNTP , 0.2 μM of each primer , 2 μl DMSO , 200 ng genomic DNA templates , and 2 units of thermostable polymerase ( proTag
from L gene sequencesdesignedfrom L gene sequences
around erroneous sequences.18designedaround erroneous sequences.18
in a specific amp ... 2011 21848932resultedin a specific amp ... 2011 21848932
in the amplification of specific Brucella DNA fragmentsresultedin the amplification of specific Brucella DNA fragments
according to ctxA gene sequencesdesignedaccording to ctxA gene sequences
from conserved sequences of RBD-2 and HBD-2designedfrom conserved sequences of RBD-2 and HBD-2
to a diverse set of genesdesignedto a diverse set of genes
for diagnosis of Lyme disease and for species - specific identification of Lyme diseasesetfor diagnosis of Lyme disease and for species - specific identification of Lyme disease