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The decrease of effective polymerase concentration due to heat inactivation and product accumulationcausedthe PCR plateau
yрutube main factorcontributingto the plateau phase in PCR
repeat numberinfluencesslippage during PCR
The factorsleadingPCR to the plateau
The DNase I treatments should be repeated for samples with a Cq value below 35to preventeffects on bispecific PCR assays
Focal seizurescausedlateralized decreases in the PCr /
In samples with a Ct value < 35 , the DNase I treatments were repeatedto preventeffects on bispecific PCR assays
The { ctrA } primersetused in the multiplex { PCR
primer depletion(passive) caused bya PCR plateau
using the Beacon Designer software 7.5 ( Primer Biosoft International , Palo Alto , CA , USA ) ( Table S4-C(passive) were designedGSPs for qRT - PCR
any mutations(passive) caused byany mutations
from too much template or too much polymeraseoften resultsfrom too much template or too much polymerase
variability and increases the standard deviationcreatesvariability and increases the standard deviation
The CCHFV - GPC insert(passive) was created byThe CCHFV - GPC insert
His - tagged paxS85A(passive) was created byHis - tagged paxS85A
GFP - tagged paxillin - S83A and paxillin - S244A constructs(passive) were created byGFP - tagged paxillin - S83A and paxillin - S244A constructs
in predicting long - term favourable outcome ... since the follow - up period is still too short , even if , to date , recurrences observed have only been in patients not experiencing a pCRwill resultin predicting long - term favourable outcome ... since the follow - up period is still too short , even if , to date , recurrences observed have only been in patients not experiencing a pCR
DNA copiesto createDNA copies
to an instable amplificationleadingto an instable amplification