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using Primer Express software version 3.0 ( Applied Biosystems , Foster City , CA(passive) were designedPrimers for qRT - PCR
using Primer Express Software Version 3.0 ( Applied Biosystems , USA(passive) were designedPrimers for qRT - PCR
on the basis of the sequences derived from the deep sequencing analysis(passive) were designedPrimers used in PCR
using the Primer Express software ( ABI(passive) were designedPrimers for qRT - PCR
using the Primer - BLAST software developed at NCBI(passive) were designedPrimers for PCR
using the PRIMER3 software ( Applied Biosystem , Massachusetts , USA(passive) were designedPrimers for the qRT - PCR
Beacon Designer 7 software ( Premier Biosoft International(passive) were designed byPrimers for qRT - PCR
where possible to overlap the site of the oligo used on the array and qRT - PCR carried out on cDNA made from RNA from the same tissue samples as used for the array experiments(passive) were designedPrimers for qRT - PCR
using Primer3 software(passive) were designedPrimers for QRT - PCR
using Primer3 software(passive) were designedPrimers for PCR
using PerlPrimer software(passive) were designedPrimers for qRT - PCR
using Primer3(passive) were designedPrimers for qRT - PCR
Use of this amount of DNAresultedin a plateau of PCR amplification product
with Primer3 software [ 30(passive) were designedPrimers for qRT - PCR
to flank each restriction site and generate PCR amplicons of 800 bp(passive) were designedPrimers for PCR
The conserved regions of plant TLPs and the N - terminal sequence were usedto designprimers for PCR
using j5 online software(passive) were designedPrimers for PCR
using the Primer3 software program ( 45(passive) were designedPrimers for PCR
using Primer3 server ( ver . 4.0.0(passive) were designedPrimers for qRT - PCR
for subsequent sequencing and analysis of cytosines within the amplicon(passive) are designedPrimers for Bisulfite PCR
using DNAstar 4.02 software(passive) were designedPrimers for the PCR
for the coding region of the UCP1 gene(passive) were designedPrimers for PCR
from the sequence of the genomic clones(passive) were designedPrimers for PCR
to amplify the consensus sequence for Bcl-2 ( F(passive) were designedPrimers for PCR
The Primer Designer helpsdesignprimers for PCR
according to the chosen DNA assembly method(passive) were designedPrimers for PCR
based on the respective published DNA sequences(passive) were designedPrimers for PCR
from the nucleotide sequences available in the art(passive) can be designedPrimers for PCR
successfully(passive) was ... designedPCR ( MAS - PCR ) genotyping system
using(passive) were designedPrimers for PCR
Any segment of the genome of T. pallidum can be usedto designprimers for PCR
To do so , I found the DNA sequence for my target proteins anddesignedprimers for PCR
In addition , ... , some prior sequence data are neededto designprimers for PCR
so as to add a recognition site for a restriction enzyme on both ends(passive) were designedPrimers for PCR
according to known algorithms(passive) can be designedPrimers for PCR
according to known algorithms(passive) may be designedPrimers for PCR
to amplify the region between -618 to +368(passive) were designedDegenerated primers for PCR
based on the sequences extracted from Online Resource for Community of Annotation of Eukaryotes(passive) were designedPrimers for qRT - PCR
from multiple databases(passive) were designedPrimers for PCR
using DNAstarTM software ( GATC Biotech , Konstanz , Germany(passive) were designedPrimers for the PCR
to increase the assay 's sensitivity and specificitywas designedto increase the assay 's sensitivity and specificity
according to the expected insertion allele of the AtYFGwill be designedaccording to the expected insertion allele of the AtYFG
to spandesignedto span
to spurious resultsledto spurious results
to include the entire coding sequence of the majority of altered genes in the whole - genome sequencing discovery cohort as well as TP53designedto include the entire coding sequence of the majority of altered genes in the whole - genome sequencing discovery cohort as well as TP53
the problems(passive) caused bythe problems
generation of stutter peakscausesgeneration of stutter peaks
in the conserved kinase domain of SERK geneswere designedin the conserved kinase domain of SERK genes
Two oligonucleotides(passive) were designedTwo oligonucleotides
to include the entire coding sequence of the majority of altered genes in the whole - genome sequencing discovery cohort as well as TP53 and in total encompassing 106.3 kB of target region ( Table S11designedto include the entire coding sequence of the majority of altered genes in the whole - genome sequencing discovery cohort as well as TP53 and in total encompassing 106.3 kB of target region ( Table S11
in artefactsresultingin artefacts
IIIsetIII
Reactions(passive) were setReactions
a repeat of the PCR at 4 - 6 weeks to see if the change is sustained and/or confirmedpromptsa repeat of the PCR at 4 - 6 weeks to see if the change is sustained and/or confirmed
A 1000-bp donor sequence(passive) was designed byA 1000-bp donor sequence
Primers(passive) were designedPrimers
in a DNA fragment of the downstream ISApl1resultingin a DNA fragment of the downstream ISApl1
Four primers(passive) were designedFour primers
alsocontributesalso
for genotypingdesignedfor genotyping
in an enriched libraryresultingin an enriched library
in a 5 ' fluorescein and 3resultedin a 5 ' fluorescein and 3
to amplify three different C. pseudotuberculosis genomic regions , including a fraction of the gene responsible for phospholipase D exotoxin expressionwas designedto amplify three different C. pseudotuberculosis genomic regions , including a fraction of the gene responsible for phospholipase D exotoxin expression
from TPMV and other hantavirusesdesignedfrom TPMV and other hantaviruses
in only very weak signals for the t=4 , t=6 , 6d+ , chi man , and chi fre samples , and three separate PCR mixturesresultedin only very weak signals for the t=4 , t=6 , 6d+ , chi man , and chi fre samples , and three separate PCR mixtures