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100 gb SG PCR Master Mix ... reaction buffer , dNTP , MgCl2 , sybrgreen and additivespreventPCR inhibition
gb Basic PCR Master Mix ... reaction buffer , dNTP , MgCl2 and additivespreventPCR inhibition
The product ... reaction buffer , dNTP , MgCl2 , sybrgreen and additivespreventPCR inhibition
calcium ions 10(passive) caused byPCR inhibition
calcium ions 432(passive) caused byPCR inhibition
calcium ions 409(passive) caused byPCR inhibition
calcium ions Lett(passive) caused byPCR inhibition
coconcentrated environmental contaminants(passive) caused byPCR inhibition
Elite PCR Master Mix ... reaction buffer , dNTP , MgCl2 and additivespreventPCR inhibition
Impurities in DNAmay resultin PCR inhibition
excessively high DNA concentrations and the need to test diluted and undiluted DNA when performing PCR assays(passive) caused byPCR inhibition
co - extracted impurities(passive) caused byPCR inhibition
complicated mixtures of organic chemicals ( for example , fatty acids , sterols , alkaloids , flavonoids , glycosides , saponins , tannins and terpenesresultin PCR inhibition
overloading the reaction with cDNA [ 11 , 22–24(passive) caused byPCR inhibition
Two microlitre of 20 mg / mL BSA solution ( Thermo Fisher Scientific ) were added to each reaction tubeto preventPCR inhibition
enoughto preventPCR inhibition
contaminant in whole blood(passive) caused byinhibition of PCR
the presence of PCR inhibitors in respiratory - tract specimens(passive) caused byPCR inhibition
The presence of any components that inhibit the Taq polymerase , such as EDTA , phenol , ethanol or humic substances in the DNA extracts ... likelyto resultin PCR inhibition
certain sample matrices and enrichment media(passive) caused byPCR inhibition
orderto preventPCR inhibition
i ) liquefactionpreventsPCR inhibition
certain types of clinical samples such as whole blood and urinemay causePCR inhibition
high salt or iron concentrations(passive) possibly caused byPCR inhibition
of each primer , 0.5 mg dried skimmed milk powder (to preventPCR inhibition
dilution of the DNA extracts ... necessaryto preventPCR inhibition
However PCR - based detection techniques ... some pharmaceutical productscan causePCR inhibition
The addition of bovine serum albumin to the reaction mixturepreventedthe inhibition of PCR
extraction contaminants in other organisms(passive) caused byPCR inhibition
Effectsresultingfrom PCR inhibition
High amounts of the cellular genomemay leadto PCR inhibition
GeneReleaser ( Bioventures , Inc. ) was added to the reaction tubesto preventPCR inhibition
placing throat swabs in Amiescausedinhibition of PCR
diffusible PC'R inhibitors from arl)NA extract of Neandertal DNA sequences and the origin of modern humans(passive) caused bythe PCR inhibition
false negativesresultingfrom PCR inhibition
It is observedcan causePCR inhibition
gold and titanium dioxide nanoparticles(passive) caused byPCR inhibition
the presence of chelating agents in the non - diluted ATAD DNA extractsmay ... contributeto the PCR inhibition
less than one genome per PCR ) targets which are encoded on genomic DNA ... eukaryotic DNAmight leadto inhibition of PCR
soil DNA extracts(passive) caused byPCR inhibition
two false - negative results(passive) apparently caused bytwo false - negative results
detection errors ( ‘ false negatives(passive) caused bydetection errors ( ‘ false negatives
negative mcyE PCR results , a PCR with cyanobacterium - specific 16S rRNA gene - targeted primers ( 32 ) was performed with DNA extracted from different - cell - size fractionscausingnegative mcyE PCR results , a PCR with cyanobacterium - specific 16S rRNA gene - targeted primers ( 32 ) was performed with DNA extracted from different - cell - size fractions
The failure to amplify nirK genes(passive) may have been caused byThe failure to amplify nirK genes
from DNA polymerase adsorptionresultingfrom DNA polymerase adsorption
from a multitude of reasons , including thermal cycler malfunction , inefficient DNA polymerase activity , incorrect PCR mixture preparation , or the presence of PCR inhibitors in the reaction mixture ( 16resultingfrom a multitude of reasons , including thermal cycler malfunction , inefficient DNA polymerase activity , incorrect PCR mixture preparation , or the presence of PCR inhibitors in the reaction mixture ( 16
in a lower amplification signal intensity levelresultingin a lower amplification signal intensity level
from residual contaminantsto resultfrom residual contaminants
Ct separately of bacterial insertmight influenceCt separately of bacterial insert
problems for detection of arbovirus when screening mosquito samples ... and that this should be taken into accountmay causeproblems for detection of arbovirus when screening mosquito samples ... and that this should be taken into account
from samples into which pure bacterial DNA was introduced before purification on a membraneoriginatefrom samples into which pure bacterial DNA was introduced before purification on a membrane
in allele dropout and peak imbalanceresultingin allele dropout and peak imbalance