Gene knockout next to introducing genes for the production of new enzymes , dna has also been introducedto creategene knockouts

While this protocol describes the use of ZFNsto createa gene knockout

small insertions or deletions ( indelsresultin gene knockout

thus causing a frame - shift mutationresultingin gene knockout

As shown in FIG . 12 , the fusion product and the S. pneumoniae genome undergo a homologous recombination event so that the erm gene replaces the chromosomal copy of the gene of interestthereby creatinga gene knockout

As shown in FIG . 26 , the fusion product and the S. pneumoniae genome undergo a homologous recombination event so that the erm gene replaces the chromosomal copy of the gene of interestthereby creatinga gene knockout

Random insertions or deletions by NHEJ in the coding region lead to frame shift mutationshence creatinggene knockouts

deletion / insertion mutations that are likelyto causegene knockout

by introducing a mutation ( e.g. , missense mutation , nonsense mutation , substitution mutation , deletion mutation , or insertion mutation ) to an open reading frame(passive) may be createdA gene knockout

selectively disabling a specific target gene in a particular type of cells called embryonic stem cells(passive) is created byThe gene knockout

an insertion of a group II intron ... a 3-hydroxyisobutyrate - dehydrogenase gene ( GR4Chr1817resultsin gene knockout

unprecedented precisionto creategene knockouts

Using zinc - finger nuclease ( ZFN ) technology , they successfully targeted the rat genomecreatinggene knockouts

the use of this technologyto creategene knockouts

Although often usedto creategene knockouts

through nonhomologous end(passive) can be createdGene knockouts

Repair DNA fragmentsto creategene knockouts

allowingto creategene knockouts

in several putative colanic acid biosynthesis genes ( wcaM , wcaA , and wza(passive) were createdGene knockouts

By using this temporal axenic amastigote culture , we successfully introduced gRNAs directly into the Cas9-expressing amastigoteto causegene knockouts

rare homozygous predicted loss of function(passive) created bygene knockouts

Unfortunately this was unsuccessful , so we opted for a simpler approachcreatinggene knockouts

to remove genes (creatinggene " knockouts

the potentialto creategene knockouts

joining ( NHEJ ) pathwayto creategene knockouts

Endogenous repair mechanisms triggered by the double - strand breakmay resultin gene knockout

DNA modification can also be used to remove genetic material from the target organismthereby creatinga gene knockout

These InDels can cause out - of - frame mutations in the coding sequences of the target genesresultingin gene knockout

to replace endogenous KCH genes with a drug - resistant marker flanked by lox - P sequences(passive) were designedGene knockout constructs

phenotypesresultingfrom gene knockout

targeted mutationsresultin gene knockout

From the NSF :To createa gene knockout

efforts underwayto creategene knockout zebrafish

package was usedto creategene knockout

Arrayitleadsan ... Gene knockout

In these animals , a wild type gene is replaced with an inactive geneto createa gene knockout

a Deletion of 8   bp within the Apoa4 exon 2 using TALEN approach causes a frameshiftresultingin gene knockout

point mutationscausinggene knockout

to induce the deletion of a particular genecreatinga gene knockout

Indel mutations occurring within the coding region of a gene can result in frameshifts and the creation of a premature stop codonresultingin gene knockout