based on the sequence of a partial cDNA for hamster Xbp1 ( supplementary material Fig(passive) were designed5′-GGTAGACCTCTGGGAGTTC-3′. Primers
less contact surface between the primer and pocketwould causeblown primers
heat(passive) caused byblown primers
placecausingblown primers
the firing pin hole for a burrwill causeblown primers
excessive pressure(passive) are caused byBlown primers
a pressure spike would occur ,causingblown primers
Excess protrusioncausesblown primers
small rifle primers to helppreventblown primers
with an algorithm implemented in a polymerase chain reaction ( PCR ) multiplexer program(passive) were designedFinder.35 Primers
in an etched bolt face on my new T3 7mm08resultedin an etched bolt face on my new T3 7mm08
to amplify from onesequence ... but not from other homologous sequencesare being designedto amplify from onesequence ... but not from other homologous sequences
I have seen and experienced what your referring to(passive) is ... caused byI have seen and experienced what your referring to
to amplify only cDNA by using an exon junction – spanning reverse primerwere designedto amplify only cDNA by using an exon junction – spanning reverse primer
to confirm the G1399A mutationdesignedto confirm the G1399A mutation
in the removal of the stop codon and introduction of XbaI and BamHI restriction sitesresultingin the removal of the stop codon and introduction of XbaI and BamHI restriction sites
The primers of objective genes(passive) were designedThe primers of objective genes
from malic acid dehydrogenase gene and their use for detection of Escherichia coli in water and milk samplesdesignedfrom malic acid dehydrogenase gene and their use for detection of Escherichia coli in water and milk samples
to span an intron and relative expression in each sampledesignedto span an intron and relative expression in each sample
Genes of interest and(passive) were designedGenes of interest and
for SYBR Green - based quantitative RT - PCRwere ... designedfor SYBR Green - based quantitative RT - PCR
to anneal to endogenous genetic materialdesignedto anneal to endogenous genetic material
to target an intronic region of the genedesignedto target an intronic region of the gene