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the transfection reagent(passive) caused byunspecific background staining
secondary antibodies(passive) caused byunspecific background staining
nonspecific binding to dead cells(passive) caused bytominimize background staining
unspecific binding of the primary antibody or the secondary antibody in the HRP - polymer(passive) caused byBackground staining
the nucleic acid probes used(passive) can be caused byBackground staining in CISH
the chromogen to precipitatethereby causingbackground staining to occur
marker amplification reagents and the effects of this oxidation on the stability of antibody - antigen complexes in immunohistochemistry(passive) caused bybackground staining
unspecific binding of the primary antibody or the secondary antibody in the AP polymer(passive) caused byBackground staining
dead cells ( stained with propidium iodide ) and B - cells (may causeunspecific background staining
any residual photosensitive layercausesbackground staining
using X - gal at pH 7.5to preventbackground staining
The chloride would react with the silver ions in the next stepcausingbackground staining
the application of serum block(passive) was prevented byBackground staining
to the variability of intensity in the target protein band between samplesleadingto the variability of intensity in the target protein band between samples
to a less likelihood of a false positive outcome ( Radulescu , Boenisch 2007resultingto a less likelihood of a false positive outcome ( Radulescu , Boenisch 2007
from autofluorescence of the layerresultingfrom autofluorescence of the layer
from the incomplete rinsing of slides between steps or by the use of contaminated bufferscan also resultfrom the incomplete rinsing of slides between steps or by the use of contaminated buffers
to false - negative or false - positive signalspotentially leadingto false - negative or false - positive signals
reliable identification of infected cells for frequencies below 0.05–0.1 % , the equivalent of 500–1000 HIVGag+ or HIVRNA+ T cells per million CD4 , when we assessed a single marker ( Figures 1EF , S2CDpreventedreliable identification of infected cells for frequencies below 0.05–0.1 % , the equivalent of 500–1000 HIVGag+ or HIVRNA+ T cells per million CD4 , when we assessed a single marker ( Figures 1EF , S2CD
from using a mouse primary antibodyresultingfrom using a mouse primary antibody
from non - specific binding of individual probesresultingfrom non - specific binding of individual probes
to a false - positive callcould leadto a false - positive call
positive staining and decrease error(passive) caused bypositive staining and decrease error
the statistical error(passive) caused bythe statistical error
from endogenous alkaline phosphatase activityresultingfrom endogenous alkaline phosphatase activity
from B cells binding to the secondary and tertiary antibodiesresultingfrom B cells binding to the secondary and tertiary antibodies