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Smart Reasoning:

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Qaagi - Book of Why

Causes

Effects

that excites the living tissueto causeautofluorescence

particulate from cells or cell culture medium and emission from YFP , any fluorescent signal larger than 5 μm(passive) caused byautofluorescence

normal mouse chow when imaging(passive) caused byautofluorescence

normal mouse chow diet when imaging(passive) caused byautofluorescence

endogenous molecules(passive) caused byautofluorescence

The excitation light cut filter blocks excitation light for exciting a living bodyto causeautofluorescence

Use a negative control to check and minimize bacterial contaminationmay causeautofluorescence

the detection chamber 170 itself ... a maximum interference materialcausesautofluorescence

the use of ade- strains as even excess adenine ... necessary sufficientto completely preventautofluorescence

While perfusion of the fixative is essential to preserve ultrastructure , reducing the concentration of glutaraldehyde is necessaryto preventautofluorescence

In these applications , usually very low concentrations ( often around 0.1 % ) of GA are usedto preventautofluorescence

the presence of fluorescent compounds , such as flavins and porphyrins(passive) may also be caused byAutofluorescence

For immunofluorescence double staining , we first added an auto - fluorescent quencher to the sectionsto preventautofluorescence

the same excitation light used to excite the fluorophore in the fluorescence assay(passive) is triggered byAutofluorescence

natural fluorescent substances occurring superficially in external tissue layers(passive) triggered byautofluorescence

necrotic plant tissue(passive) caused byautofluorescence

a shutterpreventsautofluorescence

the presence of co - enzymes in mammalian cells Emission(passive) Caused byAutofluorescence –

wavelength 390 - 440 nmcausesautofluorescence

the rounding up of cells near times of cell division(passive) caused byautofluorescence

Highly Fluorescent Disadvantages - UV excitationcausesautofluorescence

the potentialto createautofluorescence

many factors , including the age of the animal and fixation techniques(passive) is influenced byAutofluorescence

the contrast agent(passive) caused byautofluorescence

the tissue preparation and labelingis causingautofluorescence

stacking images by confocal microscopy(passive) caused byautofluorescence

the extended time in fixative(passive) caused byautofluorescence

the presence of such cellular components as elastin , lysosomes , and mitochondria among others(passive) caused byautofluorescence

cell type and physiological conditions(passive) is influenced byAutofluorescence

blood(passive) caused byautofluorescence

flavin or collagen , etc(passive) caused byautofluorescence

two - photon excitation in the green channel ( pseudocolored green(passive) caused byAutofluorescence

excitation and light- scattering signals from cellular and equipment sources(passive) caused byautofluorescence

the lipofuscin(passive) is caused byAutofluorescence

Argon LasertriggeredAutofluorescence

anti - Stokes emissionpreventsautofluorescence

the wavelength components in the ultraviolet spectrum ( UV ) of the ultraviolet - and - blue light ( UV+B ) excite living tissueto causeautofluorescence

organic solvents within the adhesivescan ... causeautofluorescence

# 5 “Over - fixationwill causeautofluorescence

the release of phenolic compounds ( Figure 3A(passive) caused byautofluorescence

to false - positive intracellular Foxp3 staining in macrophagescontributesto false - positive intracellular Foxp3 staining in macrophages

from excitation lightresultingfrom excitation light

the analytical problems(passive) caused bythe analytical problems

the light - induced reaction(passive) is created bythe light - induced reaction

to the signal observed by fluorescence microscopycontributed minimallyto the signal observed by fluorescence microscopy

problems when trying to reconstruct the cellscan causeproblems when trying to reconstruct the cells

The high signal intensity of lyophilized elastin(passive) is caused byThe high signal intensity of lyophilized elastin

the low signal - to - background contrast problem(passive) created bythe low signal - to - background contrast problem

to very high background that can make it difficult to see the signal you wantcan leadto very high background that can make it difficult to see the signal you want

a background signal to the Raman spectrum which deteriorates the signal - to - noise with which the Raman signal is detectedcan causea background signal to the Raman spectrum which deteriorates the signal - to - noise with which the Raman signal is detected

severe problems in , especially the ill - posed , fluorescence diffuse optical tomography ( FDOTcausessevere problems in , especially the ill - posed , fluorescence diffuse optical tomography ( FDOT

false - positive lesions in ovarian cancer and difficulty in discriminating breast cancer - specific fluorescence from background signalcausedfalse - positive lesions in ovarian cancer and difficulty in discriminating breast cancer - specific fluorescence from background signal

an artifact(passive) caused byan artifact

from endogenous fluorphore such as porphyrins , lipofuscin , NADPH , flavin , collagen , elastin , tryptophan , tyrosine and phenylalanine etccausedfrom endogenous fluorphore such as porphyrins , lipofuscin , NADPH , flavin , collagen , elastin , tryptophan , tyrosine and phenylalanine etc

in the center of the rosettesoriginatingin the center of the rosettes

in endogenous substances in biological tissues and the likeoriginatingin endogenous substances in biological tissues and the like

in the sampleoriginatingin the sample

from fluorescence in - situ hybridisation [ FISHresultingfrom fluorescence in - situ hybridisation [ FISH

from a varietyof fluorophores in the oral cavityoriginatesfrom a varietyof fluorophores in the oral cavity

from lipofuscin particles found in human tissueoriginatingfrom lipofuscin particles found in human tissue

within one or more components of the overall systemcreatedwithin one or more components of the overall system

from epithelial cells due tooriginatingfrom epithelial cells due to

Strong red staining in blood vessels(passive) is caused byStrong red staining in blood vessels

from the use of aldehyde fixation ( formaldehyde or glutaraldehydealso resultsfrom the use of aldehyde fixation ( formaldehyde or glutaraldehyde

from retinol within the RPEmainly originatingfrom retinol within the RPE

the visual noise(passive) caused bythe visual noise

from retinol in the retinal pigment epithelium ( RPEmainly originatingfrom retinol in the retinal pigment epithelium ( RPE

much less background(passive) caused bymuch less background

to this effect following irradiationcontributingto this effect following irradiation

from either organic or inorganic compounds or elementsmay originatefrom either organic or inorganic compounds or elements

unwanted background noise during fluorescence imaging in the visible regioncan causeunwanted background noise during fluorescence imaging in the visible region

from the perinuclear zone of live cells in culture ( Figures 6(boriginatingfrom the perinuclear zone of live cells in culture ( Figures 6(b

noises(passive) caused bynoises

from hepatocellular reduced pyridine nucleotides ( NADHresultingfrom hepatocellular reduced pyridine nucleotides ( NADH

potential false positive results(passive) caused bypotential false positive results

to our final readingscontributingto our final readings

from multilamellar bodies ( MLBs ) and secretory granulesoriginatedfrom multilamellar bodies ( MLBs ) and secretory granules

to an intense , broad background in the spectraleadsto an intense , broad background in the spectra

from aldehyde - based fixation ( Clancy and Cauller , 1998resultingfrom aldehyde - based fixation ( Clancy and Cauller , 1998

a negative control thresholdto seta negative control threshold

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Smart Reasoning:

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